Journal: Scientific Reports

Article Title: Behavioral, blood, and magnetic resonance imaging biomarkers of experimental mild traumatic brain injury

doi: 10.1038/srep28713

Figure Lengend Snippet: Rats given an mFPI had significantly increased levels of ceruloplasmin ( a ) and NF-H ( b ) in plasma on D1 compared to sham-injured rats. Additionally, mFPI rats had significantly decreased levels of tau on D7 ( c ), and VEGF on D7 and D30 ( d ) compared to their sham counterparts. There were no statistically significant differences between the groups on any of the other plasma biomarkers ( e–h ). Mean ± s.e.m., *mFPI group significantly different than sham group, p < 0.05.

Article Snippet: The primary antibodies were diluted in antibody incubation buffer (0.1% BSA, EDTA-free Halt Protease and Phosphatase Inhibitor Cocktail [Thermo Fisher Scientific], 1× TBS, and 0.5% Tween-20) and used in the following dilutions: ceruloplasmin (1:20; Santa Cruz Biotechnology, sc-21240), NF-H (1:100; Sigma–Aldrich, N4142), tau (1:100; Cell Signaling Technology, 4019), VEGF (1:50; Abcam, ab53465), 4-HNE (1:1000; EMD Millipore, 393207), NSE (1:50; Abcam, ab53025), GFAP (1:20; Abcam, ab48050), and S100β (1:20; Abcam, ab41548).

Techniques: Clinical Proteomics